The latest research and development of Diane biology GFP and mCherry nanogel / magnetic bead products Using advanced superparamagnetic beads or gels, Compared with the traditional mouse IgG monoclonal antibody, the covalently coupled high-quality Alpaca nano antibody (VHH) has higher titer and stronger protein binding ability, and has a series of advantages, such as small molecular weight (15kDa), strong stability (still stable at 70 ℃), high affinity (high specificity binding target protein), no light chain and heavy chain interference of ordinary mouse monoclonal antibody.
This series of products are mainly used for IP, CO IP RNA IP (RIP) and chip and other functional experiments. According to the use habits of different researchers, there are two experimental methods: gel method and magnetic bead method. The gel method has high load, strong enrichment ability, and magnetic bead method does not need centrifugation, which can be called immune precipitation of scientific researchers (IP / CoIP) and mass spectrometry (MS). It is committed to solving the complicated and complicated basic scientific experiment problems of scientific researchers within one hour
green fluorescent protein (GFP) It's a kind of β- The barrel protein, isolated from Aequorea victoria, consists of 238 amino acids with a molecular weight of about 27 kDa. GFP can transform the blue fluorescence of jellyfish luminescent protein into green fluorescence through energy transfer. The excitation wavelength of GFP is 488 nm and there is an emission peak at about 507 nm. At the same time, the research on the high-resolution crystal structure of GFP enables scientists to manipulate its protein structure and transform the color variants emitted at different wavelengths. Green fluorescent protein (GFP) is widely used in protein localization and protein kinetics analysis. GFP agarose or magnetic beads can provide rapid and effective one-step immunoprecipitation, which can specifically isolate GFP labeled target proteins and their interaction factors from cells and tissues. The isolated products can be used for nucleic acid analysis, protein analysis, mass spectrometry analysis, enzyme biopsy and other follow-up biophysical and chemical analysis.
Red fluorescent protein （mCherry） It is a kind of red fluorescent dye widely used as a tracer in biotechnology, including molecular labeling and cell component localization. It is different from other GFP protein and other green fluorescent protein variants isolated from Victoria jellyfish, MCherry and most of the other red fluorescent proteins come from corals The maximum excitation and emission wavelengths of mCherry are 587 nm and 610 nm, respectively. The mCherry agarose or magnetic beads can also provide a rapid and effective one-step immunoprecipitation, which can specifically isolate the target eggs labeled by mCherry from cells and tissues White and its interaction factors. The isolated products can be used for nucleic acid analysis, protein analysis, mass spectrometry analysis, enzyme biopsy and other follow-up biophysical and chemical analysis.
Results of protein purification
Enrichment, purification and elution of GFP fusion protein in E.coli lysate by GFP nanogel (product No.: ip0057s)
When the input protein was weakly expressed, GFP gel (product number: ip0057s) could enrich the target protein and enhance the IP signal. WB detection was performed by Daian biological GFP mouse monoclonal antibody (product No.: 2057)
When the expression of input protein was very weak, the mCherry gel (product No.: ip0099s) could still enrich the target protein and display the specific IP signal. WB detection was performed with the polyclonal antibody against mCherry rabbit (product No.: 3099)
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