Antibody Drug Discovery



Rat Monoclonal Antibody Service


  Our Services  


Rat monoclonal antibody can avoid immune tolerance caused by antigen homology of mouse protein. Rat McAb has PM affinity and higher specificity than mouse monoclonal antibodies. Dia-an's unique rat monoclonal antibody system, fuses activated rat B cells with tumor cells, and screens positive high specific rat monoclonal antibodies. Large-scale production of rat monoclonal antibody (ascites preparation or cell culture) can be  obtained to meet the needs of scientific researchers or industrial customers.


Our advantages:

  • Rat monoclonal antibody can solve the problem of immune tolerance caused by antigen homology of mouse protein
  • Comprehensive antigen preparation platform
  • The prepared rat McAb has PM affinity and high specificity, better than mouse McAb
  •  Ascites preparation or hybridoma cell supernatant culture can be selected to prepare purified antibody
  Service Details




Specification Timeline Deliverables Pricing

Immunogen Preparation

• Recombinant protein expression 

• Peptide synthesis and conjugation

1-4 weeks

• Design report

• Protein sample

• SDS-PAGE result





Immunization and Titer test

4-8 rats immunization
• Anti-serum titer ELISA test

8-10 weeks

Anti-serum sample

ELISA titer

Fusion & Screening

• Hybridoma fusion
• Positive clones screening
2 rounds of subcloning

4-6 week

1-3 ELISA positive clones

1-5 ml supernatant per clone

Antibody production & purification

• hybridoma culture
• ascites preparation

• Antibody purificaiton

2 week 5-10 mg antibodies per clone
QC & subtype identification


antibody subtype verification

2 week project report





  Service Process  



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