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Recombinant Antibody Expression


  Our Services  


Recombinant antibody is a monoclonal antibody expressed in vitro by gene engineering. Recombinant antibody technology makes it possible to prepare humanized antibody. And it also realize mass and stable production of monoclonal antibody without immunization. With rich experience of recombinant antibodies, Dia-an provide you with various forms of recombinant antibody services, including  antibody sequencing, recombinant antibodies expression, chimeric antibody expression, scFv, Fab, single domain antibody (sdab) and diversified Fc fusion protein expression. 


Our advantages:


● n o immunization is required, avoiding the human immune response caused by mouse monoclonal antibody

● good consistency, good repeatability of prepared antibody and small difference between batches

● recombinant transformation can be carried out, including antibody fragment expression, homotypic replacement, chimeric antibody, antibody humanization, etc

● mass preparation of antibodies: stable transfection and screening of stable cell lines are suitable for rapid industrialization of monoclonal antibody drugs 



  Service Details




Specification Timeline Deliverables Pricing
Gene synthesis 

• Gene synthesis  and codon optimization

1 week

•Expression Vector





Vector construction

• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation

1 week

Expression evaluation

• transfection into proper host cellHEK293/CHO
• small-scale transfection
•SDS-PAGE analysis to evaluate protein expression

1 week

• Expression evaluation result

Transient transfection and purification(30ml

• transfection into suspension cultured cells
• protein purification
 • SDS-PAGE and UV analysis

2 weeks

• Feasibility report
• If expression is feasible, provide a certain amount of protein sample to the client based on the expression.
Large-scale expression and purification (optional)

• 1L Flask or fermentation cylinder culture

• Affinity Purificaiton

• SDS-PAGE analysis 

Depend on scale

• Purified protein
• SDS-PAGE report
• CoA



  Our Promise  


•  The high-efficiency expression vector makes the protein more stable and with higher activity

         •  High-density suspended cells used as expression host

         •  From sequence optimization, transient transfection, to protein / antibody purification and data sorting,  only 4 weeks



  Case Studies

Recombinant Antibody Purified by Protein A Agarose

  Relative Services



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