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Custom Polyclonal Antibody Service



  Our Services  


Polyclonal antibody can recognize multiple epitopes, and has become an indispensable tool for researchers to carry out immunological research because of its short preparation schedule, low cost and wide application. Relying on the R & D and production experience of 5000 + multi antibody project, Dia-an provide polyclonal antibody preparation from rabbits, rats, mice, guinea pigs, sheep, chickens and other hosts. Various antibodies has been successfully customized against species: human, mouse, rat, zebrafish, crucian carp, grass carp, Pelteobagrus fulvidraco, mandarin fish, sea cucumber, sea urchin, nematode, Tetrahymena, bacteria, virus, Arabidopsis, cyanobacteria, cotton, corn, rice, potato, oil vegetable, wheat, etc.


Our advantages:



  • Analysis of antigen sequence, with help of professional software to design antigen, combined with literature, comprehensively select immunogen sequence, FOR FREE
  • Provide high-quality recombinant protein antigen (prokaryotic/eukaryotic),for immunization,  supernatant expression success rate > 90%, purity > 80%, concentration > 1mg / ml
  • Synthesize high-purity peptide antigen;Small molecule antigens adopt a unique activation and coupling scheme
  • The self-developed adjuvant is used to ensure that the antigen and adjuvant are fully mixed and injected subcutaneously at multiple points, so as to improve the serum titer of animals to the greatest extent


  Service Details




Specification Timeline Deliverables Pricing

Antigen analysis & design

The immunogenicity, hydrophilicity and homology of the protein were analyzed to select the appropriate specific sequence 2 days Analyisis Report  

Immunogen Preparation

Protein Route: codon optimization, gene synthesization  and soluble protein expression

3-4 weeks 100ug protein sample or 1mg peptide sample  

peptide synthesization & coupling  with carrier protein


immunize 2 rabbits or 4 rats or 5 mice

• Standard immune schedule includes 5 immunization

6-9 weeks

• 60 ml Anti-serum from 2 rabbits





Titer test

• indirect ELISA test

1 week

ELISA titer

Antibody purification

• Antigen affinity purification

1 week

5-10 mg antibodies per 10ml anti-serum



  Service Process  



    Case Studies


         Antibody titer tested by immunodiffuion                        IF verification of custom pAb


    Cited Publicaitons(Partial)

[1] Xiaobo Yu,et al. Fertilized egg cells secrete endopeptidases to avoid polytubey. Nature. 31 March.2021

[2] Zhaojing Lu ,et al. Deletion of the transmembrane protein Prom1b in zebrafish disrupts outer-segment morphogenesis and causes photoreceptor degeneration .JBC. July 30, 2019   

[3] Meng-Jie Zhao,et al. MiR-15b and miR-322 inhibit SETD3 expression to repress muscle cell differentiation [J]. Cell Death & Disease. 22 February 2019

[4] Miao Li1,2,3*, Tao Huang1,2,3*, Meng-Jing Li1,2,3*, et al. The histone modification reader   ZCWPW1 is requiredfor meiosis prophase I in male but not in female mice [J].

Science Advances 14 Aug 2019: Vol. 5, no. 8, eaax1101

[5] Functional characterization of IL-10 and its receptor subunits in a perciform fish, the mandarin fish, Siniperca chuatsi [J]. Developmental & Comparative Immunology.

Volume 97, August 2019, Pages 64-75

[6] Yao Wang1 ,et al. Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS [J].Nature Cell Biology

[7] Linlin Shi 1, Weimin Wang 1 and Yanhe Li 1,4,*et al. Molecular Characterization and Functional Study of Insulin-Like Androgenic Gland Hormone Gene   in the Red Swamp Crayfish, Procambarus clarkii[J]. MDPI Genes 2019

[8] Jing Hou, Shan Nan Chen, Zhen Gan, Nan Li, Lin Huang, Hui Jun Huo, Yue Cong Yang, Yishan Lu, Zhan Yin and Pin Nie   In Primitive Zebrafish, MHC Class II Expression Is Regulated by IFN-γ, IRF1, and Two Forms of CIITA

[9]JunyanTao, Chenglian Bai, Yuanhong Chen, Huanhuan Zhou, Yahui Liu, Qingyu Shi, WenhaoPan, Haojia Dong, Luyi Li, Hui Xu, Robyn Tanguay, Changjiang Huang, QiaoxiangDong   Environmental relevant concentrations of benzophenone-3 induced developmental neurotoxicity in zebrafish[J].Science of The Total Environment 2020

[10]Qiannan Zhang1 , Yanghong Xu1, Jishuai Huang1, Kai Zhang2, Haijun Xiao3 8 ,XiaojianQin4, Linlin Zhu5, Yingguo Zhu1,#, and Jun Hu1*   Therice pentatricopeptide repeat protein PPR756 is involved in pollen developmentby affecting multiple RNA editing in mitochondria.12 Jun 2020

[11]Qiannan Zhang1 , Yanghong Xu1, Jishuai Huang1, Kai Zhang2, Haijun Xiao3 8 ,XiaojianQin4, Linlin Zhu5, Yingguo Zhu1,#, and Jun Hu1*   The rice PPR756 coordinates with MORFsfor multiple RNA editing in mitochondria.2019

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